Difference between revisions of "Part:BBa K5301002"

(Characterization)
(Usage and Biology)
Line 9: Line 9:
 
===Usage and Biology===
 
===Usage and Biology===
  
We used this part to connect two MSP segments.
+
We employed it to link two proteins - SCSdC-mCh[1-10] and SnCSdT.
Such as
+
  
 
===Characterization===  
 
===Characterization===  

Revision as of 10:06, 30 September 2024


SdyTag can achieve covalent binding of proteins through Tag-Catcher interaction.

SdyTag is a fibronectin-binding protein. It can cooperate with SdyCatcher to achieve covalent binding of proteins through Tag-Catcher interaction. SdyTag and SdyCatcher can also be used alongside SpyTag-SpyCatcher partners, to demonstrate kinetically controlled directed protein ligation, domain specific protein circularization and macromolecular assembly [1].

Usage and Biology

We employed it to link two proteins - SCSdC-mCh[1-10] and SnCSdT.

Characterization

We used SDS-PAGE to test whether this part worked.

sds-page-of-spnw50-1-test.png

Figure 1.This is a test.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. Tan, L.L., S.S. Hoon and F.T. Wong, Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. PLOS ONE, 2016. 11(10): p. e0165074.