Difference between revisions of "Part:BBa K243027"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The sequence for the bZip domains of c-Fos is linked to a His tag | + | The sequence for the bZip domains of c-Fos is linked to a His tag and allows the purification over a Ni-NTA column and the expression as soluble proteins in ''Escherichia coli''.<br> |
In the research dimerized c-fos is normally used as a DNA binding molecule.<br> | In the research dimerized c-fos is normally used as a DNA binding molecule.<br> | ||
Revision as of 23:21, 21 October 2009
His-FOS
Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers and binds DNA as a dimer. Two classes of core DNA sequences, the sequences TRE (TGACTCA) and CRE (TGACGTCA), can be recognized by AP-1. Nine homologues of the AP-1 leucine zipper region have been characterized. All of them are able to form heterodimers, some also form homodimers. One of them is the so called c-Fos. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July).
[http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS Heterodimeric Fos complex]
Usage and Biology
The sequence for the bZip domains of c-Fos is linked to a His tag and allows the purification over a Ni-NTA column and the expression as soluble proteins in Escherichia coli.
In the research dimerized c-fos is normally used as a DNA binding molecule.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 19
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]