Difference between revisions of "Part:BBa K5415002:Design"
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− | <h5>Two sets of triple HRE containing oligonucleotide from EP0 promoter</h5> | + | <h5>Two sets of triple HRE containing oligonucleotide from EP0 promoter:</h5> |
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p> | <p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p> | ||
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p> | <p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p> | ||
<p></p> | <p></p> | ||
− | <h5>Post-EcoRV CUT</h5> | + | <h5>Post-EcoRV CUT:</h5> |
<p>gat</p> | <p>gat</p> | ||
<p></p> | <p></p> | ||
− | <h5>CYC1 promoter from pRL_yEGFP_hc</h5> | + | <h5>CYC1 promoter from pRL_yEGFP_hc:</h5> |
<p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p> | <p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p> | ||
<p></p> | <p></p> | ||
− | <h5>yeBFP2</h5> | + | <h5>yeBFP2:</h5> |
− | <p> | + | <p>ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGT</p> |
− | <p> | + | <p>GAAGGTGATGCAACAAATGGTAAATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGG</p> |
− | <p> | + | <p>GTTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATTTCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA</p> |
− | <p> | + | <p>GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAATTGAAGGGCGTTGATTTTAAGGAAGATGGTAAC</p> |
− | <p> | + | <p>ATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAAAATTAGACAT</p> |
− | <p> | + | <p>AATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT</p> |
− | <p> | + | <p>CAATCTGTTTTATCAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATAT</p> |
+ | <p>AAGTAG</p> | ||
+ | TAAGTAG</p> | ||
<p></p> | <p></p> | ||
− | <h5>ADH2 terminator</h5> | + | <h5>ADH2 terminator:</h5> |
<p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag</p> | <p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag</p> | ||
<p>ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc</p> | <p>ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc</p> | ||
<p>agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p> | <p>agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p> |
Revision as of 09:34, 30 September 2024
Contents
Two sets of triple HRE containing oligonucleotide from EP0 promoter:
TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC
TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC
Post-EcoRV CUT:
gat
CYC1 promoter from pRL_yEGFP_hc:
gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat
yeBFP2:
ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGT
GAAGGTGATGCAACAAATGGTAAATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGG
GTTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATTTCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA
GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAATTGAAGGGCGTTGATTTTAAGGAAGATGGTAAC
ATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAAAATTAGACAT
AATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT
CAATCTGTTTTATCAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATAT
AAGTAG
TAAGTAG</p>
ADH2 terminator:
gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag
ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc
agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat