Difference between revisions of "Part:BBa K5415002:Design"

 
(formatting)
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
+
<h5>Two sets of triple HRE containing oligonucleotide from EP0 promoter:</h5>
 +
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 +
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 +
<p></p>
 +
<h5>Post-EcoRV CUT:</h5>
 +
<p>gat</p>
 +
<p></p>
 +
<h5>CYC1 promoter from pRL_yEGFP_hc:</h5>
 +
<p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p>
 +
<p></p>
 +
<h5>yeBFP2:</h5>
 +
<p>ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGT</p>
 +
<p>GAAGGTGATGCAACAAATGGTAAATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGG</p>
 +
<p>GTTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATTTCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA</p>
 +
<p>GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAATTGAAGGGCGTTGATTTTAAGGAAGATGGTAAC</p>
 +
<p>ATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAAAATTAGACAT</p>
 +
<p>AATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT</p>
 +
<p>CAATCTGTTTTATCAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATAT</p>
 +
<p>AAGTAG</p>
 +
TAAGTAG</p>
 +
<p></p>
 +
<h5>ADH2 terminator:</h5>
 +
<p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag</p>
 +
<p>ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc</p>
 +
<p>agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p>

Revision as of 09:34, 30 September 2024

Two sets of triple HRE containing oligonucleotide from EP0 promoter:

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post-EcoRV CUT:

gat

CYC1 promoter from pRL_yEGFP_hc:

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeBFP2:

ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGT

GAAGGTGATGCAACAAATGGTAAATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGG

GTTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATTTCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA

GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAATTGAAGGGCGTTGATTTTAAGGAAGATGGTAAC

ATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAAAATTAGACAT

AATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT

CAATCTGTTTTATCAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATAT

AAGTAG

TAAGTAG</p>

ADH2 terminator:

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag

ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc

agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat