Difference between revisions of "Part:BBa K5102019:Design"

 
(→‎Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The 30 base pair long gRNA spacer is complementary to a synthetic ProgRAM recording tape. It features an A to C mismatch in position 22 that is a target for deamination by ADAR2 deaminase domain. The sequence also includes a gRNA loop, that is essential for the PspCas13b protein to recognize and bind the gRNA effectively.
+
The 30 base pair long gRNA spacer is complementary to a synthetic ProgRAM recording tape. It features an A to C mismatch in position 22 that is a target for deamination by ADAR2 deaminase domain. Additionally, it includes a G12>A disruption binding mismatch, that limits unspecific binding of gRNA. The sequence also includes a gRNA loop, that is essential for the PspCas13b protein to recognize and bind the gRNA effectively.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 09:13, 30 September 2024


gRNA (30bp spacer) for switching state 1 to 2 for ProgRAM (tape 1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 30 base pair long gRNA spacer is complementary to a synthetic ProgRAM recording tape. It features an A to C mismatch in position 22 that is a target for deamination by ADAR2 deaminase domain. Additionally, it includes a G12>A disruption binding mismatch, that limits unspecific binding of gRNA. The sequence also includes a gRNA loop, that is essential for the PspCas13b protein to recognize and bind the gRNA effectively.

Source

This part was ordered as a synthetic oligo from a DNA synthesis provider.

References