Difference between revisions of "Part:BBa K5235000"

Latest revision as of 09:05, 30 September 2024

BsAld

This part encodes an enzymes BsAld, from Bacillus subtilis. It is the first step in theanine synthesis, converting pyruvate to alanine. BsAld first binds with NADH to form an enzyme-cofactor complex. Then, pyruvate binds to the active site of the enzyme-cofactor complex, and NADH transfers its electrons to the pyruvate, reducing the carbonyl group (C=O) to an imine (C=NH). The imine intermediate reacts with ammonium ions to form a Schiff base intermediate, which is then hydrolyzed to produce alanine, releasing NAD⁺ and water.

[1] Cao, R., Hu, S., Lu, Y., Wang, W., Fu, Z., & Cheng, J. (2023). Fermentative Production of L-Theanine in Escherichia coli via the Construction of an Adenosine Triphosphate Regeneration System. Fermentation, 9(10), 875–875.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5235000 short</partinfo>
-This part contains two enzymes linked together. First is BsAld, from <i>Bacillus subtilis</i>, is the first step in theanine synthesis, converting pyruvate to alanine. BsAld first binds with NADH to form an enzyme-cofactor complex. Then, pyruvate binds to the active site of the enzyme-cofactor complex, and NADH transfers its electrons to the pyruvate, reducing the carbonyl group (C=O) to an imine (C=NH). The imine intermediate reacts with ammonium ions to form a Schiff base intermediate, which is then hydrolyzed to produce alanine, releasing NAD&#8314; and water.
+This part encodes an enzymes BsAld, from <i>Bacillus subtilis</i>. It is the first step in theanine synthesis, converting pyruvate to alanine. BsAld first binds with NADH to form an enzyme-cofactor complex. Then, pyruvate binds to the active site of the enzyme-cofactor complex, and NADH transfers its electrons to the pyruvate, reducing the carbonyl group (C=O) to an imine (C=NH). The imine intermediate reacts with ammonium ions to form a Schiff base intermediate, which is then hydrolyzed to produce alanine, releasing NAD&#8314; and water.
+[1] Cao, R., Hu, S., Lu, Y., Wang, W., Fu, Z., & Cheng, J. (2023). Fermentative Production of L-Theanine in Escherichia coli via the Construction of an Adenosine Triphosphate Regeneration System. Fermentation, 9(10), 875–875.
 <!-- Add more about the biology of this part here