Difference between revisions of "Part:BBa K5117021"
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<partinfo>BBa_K5117021 short</partinfo>
 
<partinfo>BBa_K5117021 short</partinfo>
  
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The promoter P<i>cotYZ<i> is located upstream the <i>cotYZ<i> operon in the <i>Bacillus subtilis<i> genome.
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This part contains the regulatory sequence of P<sub><i>cotYZ</i></sub>, which is a promoter of <i>Bacillus subtilis</i>.
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It is active during sporulation and enables sporulation dependent expression of target genes.
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P<sub><i>cotYZ</i></sub> is a sporulation-dependent promoter out of the <i>cotYZ</i> gene cluster of <i>B. subtilis</i>, which allows for gene expression only during sporulation.
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Information regarding the <i>cotYZ</i> operon can be found in the following databases:
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<html><a href="https://subtiwiki.uni-goettingen.de/">SubtiWiki</a></html>,
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<html><a href="https://dbtbs.hgc.jp/">DBTBS</a></html>.
  
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In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels <i>et al.</i> 2018, unpublished data of Elif Öztel).
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P<sub><i>cotYZ</i></sub> only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page).
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<b>Target organism:</b> <i>Bacillus subtilis</i>
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<b>Main purpose of use:</b>: Gene expression and production of fusion proteins (especially for spore surface display)
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5117021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5117021 SequenceAndFeatures</partinfo>
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===References===
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Bartels J., López Castellanos S., Radeck J., Mascher T. (2018): Sporobeads: The utilization of the <i>Bacillus subtilis</i> endospore crust as a protein display platform. ACS synthetic biology 7(2), 452-461. https://doi.org/10.1021/acssynbio.7b00285
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Öztel, E. & Mascher T. (2024): unpublished data.
  
  

Revision as of 08:45, 30 September 2024


PcotYZ

This part contains the regulatory sequence of PcotYZ, which is a promoter of Bacillus subtilis. PcotYZ is a sporulation-dependent promoter out of the cotYZ gene cluster of B. subtilis, which allows for gene expression only during sporulation. Information regarding the cotYZ operon can be found in the following databases: SubtiWiki, DBTBS.

In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels et al. 2018, unpublished data of Elif Öztel).


PcotYZ only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).


Target organism: Bacillus subtilis

Main purpose of use:: Gene expression and production of fusion proteins (especially for spore surface display)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Bartels J., López Castellanos S., Radeck J., Mascher T. (2018): Sporobeads: The utilization of the Bacillus subtilis endospore crust as a protein display platform. ACS synthetic biology 7(2), 452-461. https://doi.org/10.1021/acssynbio.7b00285

Öztel, E. & Mascher T. (2024): unpublished data.