Difference between revisions of "Part:BBa K5079013"
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<partinfo>BBa_K5079013 short</partinfo> | <partinfo>BBa_K5079013 short</partinfo> | ||
| − | + | This is the forward primer for Part:BBa_K5079032. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgtcgggaaatcccttgg. 3’ GC Content is 61%, 3' length is 18nt and 3' Tm is 68°C. | |
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Revision as of 08:42, 30 September 2024
K45_CP_P95 Switch primers into pET-28a Switch 7 (Fwd)
This is the forward primer for Part:BBa_K5079032. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgtcgggaaatcccttgg. 3’ GC Content is 61%, 3' length is 18nt and 3' Tm is 68°C.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]