Difference between revisions of "Part:BBa K5269024"

Latest revision as of 08:24, 30 September 2024

SpyTag-Glucanase-His-Tag and BsmB I cut site(for GGA)

It contains SD-sequences(BBa_K5269034), SpyTag, Endo-1,3(4)-b-glucanase(BBa_5269016) and His-Tag sequences. In addition, the BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-OmpA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, Endo-1,3(4)-b-glucanase, which hydrolyzes b-D-glucan contained in the target bacterial membrane, is expected to be stably present on the membrane vesicle, which will facilitate the introduction of shRNA into the target fungus cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 235
Illegal NotI site found at 1603
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2845
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 627
Illegal NgoMIV site found at 1252
Illegal NgoMIV site found at 1995
Illegal NgoMIV site found at 2620
Illegal AgeI site found at 385
Illegal AgeI site found at 1753
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5269024 short</partinfo>
-It contains SD sequences(BBa_K5269034), SpyTag, Endo-1,3(4)-beta-glucanase(BBa_5269016) and 6xHis sequences. The SpyTag/SpyCatcher system works when used with Lpp-ompA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, Endo-1,3(4)-beta-glucanase, which hydrolyzes beta-D-glucan contained in the target bacterial membrane, is expected to be stably present on the membrane vesicle, which will facilitate the introduction of shRNA into the target fungus cells.
+It contains SD-sequences(BBa_K5269034), SpyTag, Endo-1,3(4)-b-glucanase(BBa_5269016) and His-Tag sequences.  In addition, the BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-OmpA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, Endo-1,3(4)-b-glucanase, which hydrolyzes b-D-glucan contained in the target bacterial membrane, is expected to be stably present on the membrane vesicle, which will facilitate the introduction of shRNA into the target fungus cells.
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