Difference between revisions of "Part:BBa K5117021"
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<partinfo>BBa_K5117021 short</partinfo>
 
<partinfo>BBa_K5117021 short</partinfo>
  
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The promoter P<i>cotYZ</i> is located upstream the <i>cotYZ</i> operon in the <i>Bacillus subtilis</i> genome.
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The promoter P<sub><i>cotYZ</i></sub> is located upstream the <i>cotYZ</i> operon in the <i>Bacillus subtilis</i> genome.
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It is active during sporulation and enables sporulation dependent expression of target genes.
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It is active during sporulation and enables sporulation-dependent expression of target genes.
  
  
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<!-- Add more about the biology of this part here
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P<sub><i>cotYZ</i></sub> is a sporulation-dependent promoter out of the <i>cotYZ</i> gene cluster of <i>B. subtilis</i>, which allows for gene expression only during sporulation. In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels <i>et al.</i> 2018, unpublished data of Elif Öztel).
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===Usage and Biology===
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P<sub><i>cotYZ</i></sub> only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page).
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<b>Target organism:</b> <i>Bacillus subtilis</i>
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<b>Main purpose of use:</b>: Gene expression and production of fusion proteins (especially for spore surface display)
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5117021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5117021 SequenceAndFeatures</partinfo>
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===References===
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Bartels J., López Castellanos S., Radeck J., Mascher T. (2018): Sporobeads: The utilization of the <i>Bacillus subtilis</i> endospore crust as a protein display platform. ACS synthetic biology 7(2), 452-461. https://doi.org/10.1021/acssynbio.7b00285
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Öztel, E. & Mascher T. (2024): unpublished data.
  
  

Revision as of 07:54, 30 September 2024


PcotYZ

The promoter PcotYZ is located upstream the cotYZ operon in the Bacillus subtilis genome. It is active during sporulation and enables sporulation-dependent expression of target genes.


PcotYZ is a sporulation-dependent promoter out of the cotYZ gene cluster of B. subtilis, which allows for gene expression only during sporulation. In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels et al. 2018, unpublished data of Elif Öztel).

PcotYZ only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).


Target organism: Bacillus subtilis Main purpose of use:: Gene expression and production of fusion proteins (especially for spore surface display)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Bartels J., López Castellanos S., Radeck J., Mascher T. (2018): Sporobeads: The utilization of the Bacillus subtilis endospore crust as a protein display platform. ACS synthetic biology 7(2), 452-461. https://doi.org/10.1021/acssynbio.7b00285

Öztel, E. & Mascher T. (2024): unpublished data.