Difference between revisions of "Part:BBa K5117020:Design"
 
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removal of restriction sites which are used for RFC[10] or Type IIS standards,  
 
removal of restriction sites which are used for RFC[10] or Type IIS standards,  
  
−
addition of a signal peptide SP<sub<<i>aprE</i></sub> for secretion in <i>Bacillus subtilis</i>,  
+
addition of a signal peptide SP<sub><i>aprE</i></sub> for secretion in <i>Bacillus subtilis</i>,  
  
 
addition of start codon (ATG) and stop codon (TAA)
 
addition of start codon (ATG) and stop codon (TAA)
−
 
  
 
===Source===
 
===Source===
 
<i>Bacterium HR29</i>
 
<i>Bacterium HR29</i>

Latest revision as of 07:04, 30 September 2024


BsRBS-BhrPET


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 594
    Illegal AgeI site found at 639
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,

addition of a signal peptide SPaprE for secretion in Bacillus subtilis,

addition of start codon (ATG) and stop codon (TAA)

Source

Bacterium HR29