Difference between revisions of "Part:BBa K5117017:Design"
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
removal of restriction sites which are used for RFC10 or TypeIIS standards,  
+
removal of restriction sites which are used for RFC[10] or Type IIS standards,  
 +
 
 +
 
 
exchange of start codon (GTG > ATG) for usage in <i>Bacillus subtilis</i> as host organism
 
exchange of start codon (GTG > ATG) for usage in <i>Bacillus subtilis</i> as host organism
  
 
===Source===
 
===Source===
 
<i>Bacillus halodurans</i>
 
<i>Bacillus halodurans</i>
−
 
−
===References===
 

Revision as of 07:02, 30 September 2024


BsRBS-BhBglA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 579
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,


exchange of start codon (GTG > ATG) for usage in Bacillus subtilis as host organism

Source

Bacillus halodurans