Difference between revisions of "Part:BBa K5071015"

 
 
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pACYCDuet-1 backbone
 
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===Usage and Biology===
 
  
 
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===Functional Parameters===
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    <h2>Composite part BBa_K5071015 (pACYCDuet-1 backbone)</h2>
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    <h3>Name: pACYCDuet-1 backbone</h3>
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    <p><strong>Base Pairs:</strong> 3766 bp</p>
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    <p><strong>Origin:</strong> Escherichia coli</p>
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    <h3>Usage and Biology</h3>
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        The pACYCDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pACYCD, and we used this vector for the connection of the target genes.
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    <h3>Cultivation</h3>
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        The pACYCDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pACYCD, with a length of 3766 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid.
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        <img src="https://static.igem.wiki/teams/5071/bba-k5071015/1.jpg" alt="Fig 1. The purpose segment of backbone pACYCDuet-1">
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        <div class="caption">Fig 1. The purpose segment of backbone pACYCDuet-1</div>
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Latest revision as of 05:41, 30 September 2024


pACYCDuet-1 backbone


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 1752
    Illegal NotI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 305
    Illegal BamHI site found at 106
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 324
    Illegal AgeI site found at 566
    Illegal AgeI site found at 1838
    Illegal AgeI site found at 2161
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


BBa_K5071015 (pACYCDuet-1 backbone)

Composite part BBa_K5071015 (pACYCDuet-1 backbone)

Name: pACYCDuet-1 backbone

Base Pairs: 3766 bp

Origin: Escherichia coli

Usage and Biology

The pACYCDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pACYCD, and we used this vector for the connection of the target genes.

Cultivation

The pACYCDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pACYCD, with a length of 3766 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid.

Fig 1. The purpose segment of backbone pACYCDuet-1
Fig 1. The purpose segment of backbone pACYCDuet-1