Difference between revisions of "Part:BBa K5291010"
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===Usage and Biology===
 
===Usage and Biology===
  
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The gene <i>hok</i>/<i>mok</i> can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system.<br><br>
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The gene <i>hok</i>/<i>mok</i> from <i>hok</i>/<i>sok</i> system can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system. And <i>sok</i>, the gene also in <i>hok</i>/<i>sok</i> system, can encode a kind of antisense RNA to specifically bind to <i>hok</i> mRNA, repressing the expression of toxin.<br><br>
 
<html><img width = "800" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksoknew.png" /></html><br>
 
<html><img width = "800" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksoknew.png" /></html><br>
 
<b>Fig.1 The schema of <i>hok</i>/<i>mok</i> (the gene on the right)</b><br><br>
 
<b>Fig.1 The schema of <i>hok</i>/<i>mok</i> (the gene on the right)</b><br><br>
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<html><img width = "800" src="" /></html><br>
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<html><img width = "800" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksok-normal.png" /></html><br>
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<b>Fig.2 The mechanism of <i>hok</i>/<i>sok</i> system</b><br>
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<html><img width = "400" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksok-pcr-arm2.png" /></html><br>
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<b>Fig.3 The AGE figure of <i>hok</i>/<i>sok</i></b><br><br>
 
For convenience, we set <i>hok</i>/<i>mok</i> and <i>sok</i> as a whole and didn't prepare both of them separately.<br><br>
 
For convenience, we set <i>hok</i>/<i>mok</i> and <i>sok</i> as a whole and didn't prepare both of them separately.<br><br>
  

Revision as of 04:34, 30 September 2024


hok/mok

The gene encoding a kind of toxic protein that can lead to bacteria death, whose mRNA will be repressed by sok antisense RNA.


Usage and Biology

The gene hok/mok from hok/sok system can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system. And sok, the gene also in hok/sok system, can encode a kind of antisense RNA to specifically bind to hok mRNA, repressing the expression of toxin.


Fig.1 The schema of hok/mok (the gene on the right)


Fig.2 The mechanism of hok/sok system

Fig.3 The AGE figure of hok/sok

For convenience, we set hok/mok and sok as a whole and didn't prepare both of them separately.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]