Difference between revisions of "Part:BBa K5520002:Design"

 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 +
__NOTOC__
 +
<partinfo>BBa_K5520002 short</partinfo>
  
 +
<partinfo>BBa_K5520002 SequenceAndFeatures</partinfo>
 +
 +
 +
===Design Notes===
 +
In designing an experiment to express and purify CDA protein by the PET-28a system, the inclusion of a 6×His tag was intended to facilitate subsequent protein purification and identification.
 +
 +
 +
 +
===Source===
 +
 +
Escherichia coli and Bacillus pumilus
 +
 +
===References===

Latest revision as of 02:31, 30 September 2024

6*His-CDA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 544


Design Notes

In designing an experiment to express and purify CDA protein by the PET-28a system, the inclusion of a 6×His tag was intended to facilitate subsequent protein purification and identification.


Source

Escherichia coli and Bacillus pumilus

References