Difference between revisions of "Part:BBa K5520002"
 
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<partinfo>BBa_K5520002 short</partinfo>
 
<partinfo>BBa_K5520002 short</partinfo>
  
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It encodes a chitosanase, a class of glycoside hydrolases that specifically degrade chitosan. They convert high molecular weight chitosan into low molecular weight functional chito-oligosaccharides by disrupting the &#946;-1,4-glycosidic bond between aminoglucans.
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This part is composed of 6×His tag derived from a plasmid vector in the E. coli expression system and BBa_K5520000 derived from Bacillus pumilus strain. The 6×His tag is a commonly used protein tag consisting of six histidine (His) residues that is commonly used for CDA protein isolation and purification.  
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 02:18, 30 September 2024


6*His-CDA

This part is composed of 6×His tag derived from a plasmid vector in the E. coli expression system and BBa_K5520000 derived from Bacillus pumilus strain. The 6×His tag is a commonly used protein tag consisting of six histidine (His) residues that is commonly used for CDA protein isolation and purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 544