Difference between revisions of "Part:BBa K5324015"

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<partinfo>BBa_K5324015 short</partinfo>
 
<partinfo>BBa_K5324015 short</partinfo>
  
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This part is the Destructin-1 Expression Device that lets us see if Destructin-1 is active at the end of our sequence. The Destructin-1 (BBa_K5324015) is inserted into the circuit along with a His-tag (K112703) to see if Destructin-1 is still present at the end of the sequence. This device is enriched with a promoter, RBS, Short Linker, and terminator.
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This composite part produces destructin-1, a collagen degrading serine protease normally produced by P. destructans. The destructin-1 sequence on its C-terminal has a histidine purification tag connected with a linker. The composite is reinforced with an Anderson promoter, RBS, and terminator.
  
 
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Revision as of 01:02, 30 September 2024


Destructin-1 Expression Device

This composite part produces destructin-1, a collagen degrading serine protease normally produced by P. destructans. The destructin-1 sequence on its C-terminal has a histidine purification tag connected with a linker. The composite is reinforced with an Anderson promoter, RBS, and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 102
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]