Difference between revisions of "Part:BBa K5324017"

 
 
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<partinfo>BBa_K5324017 short</partinfo>
 
<partinfo>BBa_K5324017 short</partinfo>
  
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Filler
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This composite part produces and secretes TEVp, a highly specific cysteine protease. The TEVp sequence on its N-terminal has a histidine purification tag connected with a linker. On the C-terminal is the Hly-A secretion signal marking the protein for secretion through the type 1 secretion pathway, which is connected with a TEV signal peptide sequence that is recognized by the TEV protease to be cleaved after secretion. The composite is reinforced with an Anderson promoter, RBS, and terminator.
  
 
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Latest revision as of 00:52, 30 September 2024


TEVp Secretion Device

This composite part produces and secretes TEVp, a highly specific cysteine protease. The TEVp sequence on its N-terminal has a histidine purification tag connected with a linker. On the C-terminal is the Hly-A secretion signal marking the protein for secretion through the type 1 secretion pathway, which is connected with a TEV signal peptide sequence that is recognized by the TEV protease to be cleaved after secretion. The composite is reinforced with an Anderson promoter, RBS, and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 600
    Illegal NgoMIV site found at 906
    Illegal AgeI site found at 261
  • 1000
    COMPATIBLE WITH RFC[1000]