Difference between revisions of "Part:BBa K5324011"
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<partinfo>BBa_K5324011 short</partinfo> | <partinfo>BBa_K5324011 short</partinfo> | ||
| − | This part | + | This composite part produces and secretes aprotinin, a serine protease inhibitor. The aprotinin sequence on its N-terminal has a histidine purification tag connected with a linker. On the C-terminal is the Hly-A secretion signal marking the protein for secretion through the type 1 secretion pathway, which is connected with a TEV signal peptide sequence that is recognized by the TEV protease to be cleaved after secretion. The composite is reinforced with an Anderson promoter, RBS, and terminator. |
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Latest revision as of 00:42, 30 September 2024
Aprotinin Secretion Device
This composite part produces and secretes aprotinin, a serine protease inhibitor. The aprotinin sequence on its N-terminal has a histidine purification tag connected with a linker. On the C-terminal is the Hly-A secretion signal marking the protein for secretion through the type 1 secretion pathway, which is connected with a TEV signal peptide sequence that is recognized by the TEV protease to be cleaved after secretion. The composite is reinforced with an Anderson promoter, RBS, and terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]