Difference between revisions of "Part:BBa K5324015"
m |
|||
Line 3: | Line 3: | ||
<partinfo>BBa_K5324015 short</partinfo> | <partinfo>BBa_K5324015 short</partinfo> | ||
− | This part is the Destructin-1 Expression Device that lets us see if Destructin-1 is active at the end of our sequence. The Destructin-1 (BBa_K5324015) is inserted into the circuit along with a His-tag (K112703) to see if Destructin-1 is still present at the end of the sequence. This device is enriched with a promoter, | + | This part is the Destructin-1 Expression Device that lets us see if Destructin-1 is active at the end of our sequence. The Destructin-1 (BBa_K5324015) is inserted into the circuit along with a His-tag (K112703) to see if Destructin-1 is still present at the end of the sequence. This device is enriched with a promoter, RBS, Short Linker, and terminator. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 00:11, 30 September 2024
Destructin-1 Expression Device
This part is the Destructin-1 Expression Device that lets us see if Destructin-1 is active at the end of our sequence. The Destructin-1 (BBa_K5324015) is inserted into the circuit along with a His-tag (K112703) to see if Destructin-1 is still present at the end of the sequence. This device is enriched with a promoter, RBS, Short Linker, and terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 102 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 879
- 1000COMPATIBLE WITH RFC[1000]