Difference between revisions of "Part:BBa K5089007:Design"

 
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===Design Notes===
 
===Design Notes===
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This expression system is codon optimized for expression in *E.coli*.  
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This expression system is codon optimized for expression in E.coli.
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−
 
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===Source===
 
===Source===
  
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MGS0156 is a serine-dependent ⍺/β hydrolase originally identified from an environmental metagenomic analysis study to look for enzymes to degrade PLA[1]. LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene, polypropylene and polylactic acid [2]. CsgA is a protein component of *E.coil* biofilms, which can be used as an anchor to surface display proteins [3].  
+
MGS0156 is a serine-dependent ⍺/β hydrolase originally identified from an environmental metagenomic analysis study to look for enzymes to degrade PLA[1]. LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene, polypropylene and polylactic acid [2]. CsgA is a protein component of E.coli biofilms, which can be used as an anchor to surface display proteins [3].
  
 
===References===
 
===References===
 +
1. Hajighasemi, M., et al., 2016. Biochemical and Structural Insights into Enzymatic. Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases. Biomacromolecules, 17(6), 2027–2039. https://doi.org/10.1021/acs.biomac.6b00223
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2. Lu, Y., Hintzen, K.-W., Kurkina, T., Ji, Y., and Schwaneberg, U. 2023. Directed Evolution of Material Binding Peptide for Polylactic Acid-specific Degradation in Mixed Plastic Wastes. ACS Catalysis, 13(19), 12746–12754. https://doi.org/10.1021/acscatal.3c02142
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 +
3. Fei Li, Luona Ye, Longyu Zhang, Xiaoyan Li, Xiaoxiao Liu, Jiarui Zhu, Huanhuan Li, Huimin Pang, Yunjun Yan, Li Xu, Min Yang, Jinyong Yan, Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive, Materials Today Bio, Volume 14, 2022.

Latest revision as of 20:22, 29 September 2024


MGS0156-CsgA-LCI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1059
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 159
    Illegal NgoMIV site found at 330
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 843


Design Notes

This expression system is codon optimized for expression in E.coli.

Source

MGS0156 is a serine-dependent ⍺/β hydrolase originally identified from an environmental metagenomic analysis study to look for enzymes to degrade PLA[1]. LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene, polypropylene and polylactic acid [2]. CsgA is a protein component of E.coli biofilms, which can be used as an anchor to surface display proteins [3].

References

1. Hajighasemi, M., et al., 2016. Biochemical and Structural Insights into Enzymatic. Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases. Biomacromolecules, 17(6), 2027–2039. https://doi.org/10.1021/acs.biomac.6b00223

2. Lu, Y., Hintzen, K.-W., Kurkina, T., Ji, Y., and Schwaneberg, U. 2023. Directed Evolution of Material Binding Peptide for Polylactic Acid-specific Degradation in Mixed Plastic Wastes. ACS Catalysis, 13(19), 12746–12754. https://doi.org/10.1021/acscatal.3c02142

3. Fei Li, Luona Ye, Longyu Zhang, Xiaoyan Li, Xiaoxiao Liu, Jiarui Zhu, Huanhuan Li, Huimin Pang, Yunjun Yan, Li Xu, Min Yang, Jinyong Yan, Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive, Materials Today Bio, Volume 14, 2022.