Difference between revisions of "Part:BBa K5035004:Design"

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A0A419DFF0 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Dehalococcoidia bacterium. A0A419DFF0 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A419DFF0's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities.
 
A0A419DFF0 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Dehalococcoidia bacterium. A0A419DFF0 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A419DFF0's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities.
  
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===Design Notes===
 
A hexahistidine tag for nickel column purification was added to A0A419DFF0.
 
A hexahistidine tag for nickel column purification was added to A0A419DFF0.
  
References:
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===References===
 
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https://www.uniprot.org/uniprotkb/A0A419DFF0/entry
 
https://www.uniprot.org/uniprotkb/A0A419DFF0/entry

Revision as of 18:11, 29 September 2024

A0A419DFF0 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Dehalococcoidia bacterium. A0A419DFF0 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A419DFF0's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities.

Design Notes

A hexahistidine tag for nickel column purification was added to A0A419DFF0.

References

https://www.uniprot.org/uniprotkb/A0A419DFF0/entry