Difference between revisions of "Part:BBa K5035005:Design"
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A0A2E5NSL8 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Acidimicrobiaceae bacterium. A0A2E5NSL8 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A2E5NSL8's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities. | A0A2E5NSL8 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Acidimicrobiaceae bacterium. A0A2E5NSL8 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A2E5NSL8's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities. | ||
+ | ===Design Notes=== | ||
A hexahistidine tag for nickel column purification was added to A0A2E5NSL8. | A hexahistidine tag for nickel column purification was added to A0A2E5NSL8. | ||
− | References | + | ===References=== |
− | + | ||
https://www.uniprot.org/uniprotkb/A0A2E5NSL8/publications | https://www.uniprot.org/uniprotkb/A0A2E5NSL8/publications |
Revision as of 18:11, 29 September 2024
A0A2E5NSL8 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Acidimicrobiaceae bacterium. A0A2E5NSL8 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A2E5NSL8's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities.
Design Notes
A hexahistidine tag for nickel column purification was added to A0A2E5NSL8.