Difference between revisions of "Part:BBa K5499008"

 
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===Usage and Biology===
 
===Usage and Biology===
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1. Self-Cleaving Mechanism:
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P2A peptide has a specific amino acid sequence that enables it to separate two proteins at the end of translation through an intrinsic self-cleavage mechanism. The process occurs as follows:
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·When translating to the P2A peptide, a transient peptide chain is produced.
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·At the C-terminus of the P2A peptide, an amide bond breaks, resulting in two independent proteins.
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·This cleavage process is enzymatic and does not rely on external enzymes.
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2. Translation Efficiency:
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The cleavage efficiency of P2A is very high, typically exceeding 90%, meaning most translated mRNA successfully produces two functional proteins. This high efficiency makes P2A widely applicable in genetic engineering.
  
 
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Revision as of 16:42, 29 September 2024


P2A is a self-cleaving peptide

P2A is a self-cleaving peptide derived from Porcine teschovirus-1, a short peptide (typically consisting of 18-22 amino acids) that mediates the cleavage of polyproteins during translation in eukaryotic cells. The P2A peptide is highly useful in gene therapy and protein expression because it can efficiently cleave multiple gene products into mature proteins without the need for proteases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]