Difference between revisions of "Part:BBa K5108002:Design"

 
 
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===Design Notes===
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<html>
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Native nucleotidic sequence from Pseudomonas fluorescens SBW25
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<h2>Design Notes</h2>
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<p>All BioBrick parts used for this part are compatible with the RFC10 Biobrick standard. Fusion of basic parts was performed by In-Fusion Assembly (Takara, France).</p>
  
  
  
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===Source===
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<h2>Source</h2>
  
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Uniprot C3K2E2 (https://www.uniprot.org/uniprotkb/C3K2E2/entry)
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<p>Isolated from <i>Pseudomonas fluorescens</i> SBW25. Primers for genomic amplification:<br>
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FWD: 5’-ggccgcggccgcgcgaattcaaaaaaaccagaggaaaccctgatg-3’<br>
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REV: 5’-cacgacgcggccgcaagctttcagtcagtcagtttttcagcca-3’</p>
  
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===References===
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<h2>References</h2>
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<a href="https://www.uniprot.org/uniprotkb/C3K2E2/entry" target="blank">Uniprot C3K2E2</a>

Latest revision as of 16:05, 29 September 2024


Catalase from Pseudomonas fluorescens SBW25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 917
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 148
    Illegal NgoMIV site found at 322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 723
    Illegal SapI.rc site found at 1240


Design Notes

All BioBrick parts used for this part are compatible with the RFC10 Biobrick standard. Fusion of basic parts was performed by In-Fusion Assembly (Takara, France).

Source

Isolated from Pseudomonas fluorescens SBW25. Primers for genomic amplification:
FWD: 5’-ggccgcggccgcgcgaattcaaaaaaaccagaggaaaccctgatg-3’
REV: 5’-cacgacgcggccgcaagctttcagtcagtcagtttttcagcca-3’

References

Uniprot C3K2E2