Difference between revisions of "Part:BBa K5109020"

Latest revision as of 15:41, 29 September 2024

Dehalogenase S surface display system

This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with Dehalogenase S (BBa_K5109013). This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence.

Usage and Biology

This surface display system aims to express the dehalogenase on the outer side of the cell membrane. This way, we could test it's functioning on PFAS molecules directly on the external medium, without stressing or damaging E. coli by expressing the enzyme inside.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 639
Illegal BamHI site found at 1582
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 667

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5109020 short</partinfo>
-This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with DehaS (BBa_K5109013).
+This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with Dehalogenase S (BBa_K5109013).
 This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme.
 To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence.