Difference between revisions of "Part:BBa K5477016"
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In particular, UGT2B15 is responsible for the glucuronidation of a wide range of compounds, including bisphenol A (BPA), steroid hormones (like androgens), and pharmaceuticals. By conjugating these substances with glucuronic acid, UGT2B15 facilitates their removal via urine or bile, reducing their biological activity and toxicity. The enzyme is expressed predominantly in the liver but is also found in other tissues such as the kidney, prostate, and gastrointestinal tract, where it participates in localized detoxification processes (1). | In particular, UGT2B15 is responsible for the glucuronidation of a wide range of compounds, including bisphenol A (BPA), steroid hormones (like androgens), and pharmaceuticals. By conjugating these substances with glucuronic acid, UGT2B15 facilitates their removal via urine or bile, reducing their biological activity and toxicity. The enzyme is expressed predominantly in the liver but is also found in other tissues such as the kidney, prostate, and gastrointestinal tract, where it participates in localized detoxification processes (1). | ||
| − | By incorporating UGT2B15 into the detoxification system, the goal is to metabolize BPA through glucuronidation, rendering it less harmful and easier to excrete from the cell or organism. | + | By incorporating UGT2B15 into the detoxification system [https://parts.igem.org/Part:BBa_K5477046 | BBa_K5477046], the goal is to metabolize BPA through glucuronidation, rendering it less harmful and easier to excrete from the cell or organism. |
| − | In our system, UGT2B15 was integrated in the following composites together with UDPD: | + | In our system, UGT2B15 was integrated in the following composites together with UDPD: [https://parts.igem.org/Part:BBa_K5477035 | BBa_K5477035] and [https://parts.igem.org/Part:BBa_K5477040 | BBa_K5477040]. |
Revision as of 15:35, 29 September 2024
UGT2B15 - UDP-glucuronosyltransferase 2B15
UGT2B15 (UDP-glucuronosyltransferase 2B15) is an enzyme belonging to the UDP-glucuronosyltransferase (UGT) family, which plays a role in the phase II metabolism of xenobiotics and endogenous compounds. UGT2B15 is primarily involved in the process of glucuronidation, a biochemical reaction where glucuronic acid is transferred to lipophilic molecules, making them more water-soluble and easier to excrete from the body. This detoxification process is essential for eliminating harmful substances such as drugs, environmental toxins, and metabolic byproducts.
In particular, UGT2B15 is responsible for the glucuronidation of a wide range of compounds, including bisphenol A (BPA), steroid hormones (like androgens), and pharmaceuticals. By conjugating these substances with glucuronic acid, UGT2B15 facilitates their removal via urine or bile, reducing their biological activity and toxicity. The enzyme is expressed predominantly in the liver but is also found in other tissues such as the kidney, prostate, and gastrointestinal tract, where it participates in localized detoxification processes (1).
By incorporating UGT2B15 into the detoxification system | BBa_K5477046, the goal is to metabolize BPA through glucuronidation, rendering it less harmful and easier to excrete from the cell or organism.
In our system, UGT2B15 was integrated in the following composites together with UDPD: | BBa_K5477035 and | BBa_K5477040.
Results from Drylab
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1342
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1342
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1342
Illegal BamHI site found at 1162
Illegal BamHI site found at 1456 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1342
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1342
- 1000COMPATIBLE WITH RFC[1000]
References
1. Ramírez, Viviana & Gálvez Ontiveros, Yolanda & Porras, Patricia & Martinez-Gonzalez, Luis & Rivas, Ana & Alvarez-Cubero, María. (2021). METABOLIC pathways, alterations in MIRNAS expression and effects of genetic polymorphisms of bisphenol a analogues: A SYSTEMATIC review. Environmental Research. 197. 111062. 10.1016/j.envres.2021.111062.