Difference between revisions of "Part:BBa K5280129:Design"

 
 
Line 1: Line 1:
 +
__NOTOC__
 +
<partinfo>BBa_K5280129 short</partinfo>
  
 +
<partinfo>BBa_K5280129 SequenceAndFeatures</partinfo>
 +
 +
 +
===Design Notes===
 +
We did reverse translate and make sure it is iGEM Type II compatible.
 +
 +
===Source===
 +
 +
The sequence was firstly published in Li's paper in 2020.
 +
 +
===References===
 +
Li, X., Zhang, C., Xu, X., Miao, J., Yao, J., Liu, R., Zhao, Y., Chen, X., & Yang, Y. (2020). A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells. Nucleic acids research, 48(6), e33. https://doi.org/10.1093/nar/gkaa044

Latest revision as of 15:28, 29 September 2024

LexRO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
    Illegal NotI site found at 587
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We did reverse translate and make sure it is iGEM Type II compatible.

Source

The sequence was firstly published in Li's paper in 2020.

References

Li, X., Zhang, C., Xu, X., Miao, J., Yao, J., Liu, R., Zhao, Y., Chen, X., & Yang, Y. (2020). A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells. Nucleic acids research, 48(6), e33. https://doi.org/10.1093/nar/gkaa044