Difference between revisions of "Part:BBa K5301001"
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SdyCatcher is a fibronectin-binding protein. It can cooperate with SdyTag to achieve covalent binding of proteins through Tag-Catcher interaction. SdyCatcher and SdyTag can also be used alongside SpyTag-SpyCatcher partners, to demonstrate kinetically controlled directed protein ligation, domain specific protein circularization and macromolecular assembly <ref>Tan, L.L., S.S. Hoon and F.T. Wong, Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. PLOS ONE, 2016. 11(10): p. e0165074.</ref>. | SdyCatcher is a fibronectin-binding protein. It can cooperate with SdyTag to achieve covalent binding of proteins through Tag-Catcher interaction. SdyCatcher and SdyTag can also be used alongside SpyTag-SpyCatcher partners, to demonstrate kinetically controlled directed protein ligation, domain specific protein circularization and macromolecular assembly <ref>Tan, L.L., S.S. Hoon and F.T. Wong, Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. PLOS ONE, 2016. 11(10): p. e0165074.</ref>. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We used this part to. | + | We used this part to connect two MSP segments.. |
===Characterization=== | ===Characterization=== | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 14:59, 29 September 2024
SdyCatcher can achieve covalent binding of proteins through Tag-Catcher interaction.
SdyCatcher is a fibronectin-binding protein. It can cooperate with SdyTag to achieve covalent binding of proteins through Tag-Catcher interaction. SdyCatcher and SdyTag can also be used alongside SpyTag-SpyCatcher partners, to demonstrate kinetically controlled directed protein ligation, domain specific protein circularization and macromolecular assembly [1].
Usage and Biology
We used this part to connect two MSP segments..
Characterization
We used SDS-PAGE to test whether this part worked.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 10
Illegal AgeI site found at 58 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 55
Illegal BsaI.rc site found at 256
- ↑ Tan, L.L., S.S. Hoon and F.T. Wong, Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. PLOS ONE, 2016. 11(10): p. e0165074.