Difference between revisions of "Part:BBa K5291010"
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The gene encoding a kind of toxic protein that can lead to bacteria death, whose mRNA will be repressed by <i>sok</i> antisense RNA. | The gene encoding a kind of toxic protein that can lead to bacteria death, whose mRNA will be repressed by <i>sok</i> antisense RNA. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | The gene <i>hok</i>/<i>mok</i> can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system.<br><br> | ||
+ | <html><img width = "800" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksoknew.png" /></html><br> | ||
+ | <b>Fig.1 The schema of <i>hok</i>/<i>mok</i> (the gene on the right)</b><br><br> | ||
+ | <html><img width = "800" src="" /></html><br> | ||
+ | For convenience, we set <i>hok</i>/<i>mok</i> and <i>sok</i> as a whole and didn't prepare both of them separately.<br><br> | ||
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Revision as of 14:36, 29 September 2024
hok/mok
The gene encoding a kind of toxic protein that can lead to bacteria death, whose mRNA will be repressed by sok antisense RNA.
Usage and Biology
The gene hok/mok can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system.
Fig.1 The schema of hok/mok (the gene on the right)
For convenience, we set hok/mok and sok as a whole and didn't prepare both of them separately.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]