Difference between revisions of "Part:BBa K5108003:Design"

 
 
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===Design Notes===
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Codon optimized nucleotidic sequence from Pseudomonas putida adapted to Pseudomonas putida is obtained thanks to the Codon Optimization Tool from Integrated DNA Technologies (https://eu.idtdna.com/pages/tools/codon-optimization-tool?returnurl=%2FCodonOpt). This step permits to lower complexity and minimize secondary structures.
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<h2 style="color: blue;">Sources</h2>
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<p>The codon-optimized nucleotide sequence from <i>Pseudomonas putida</i> adapted to <i>Pseudomonas putida</i> was obtained using the <a href="https://eu.idtdna.com/pages/tools/codon-optimization-tool?returnurl=%2FCodonOpt" target="blank">Codon Optimizer Tool</a> from Integrated DNA Technologies. This step permits optimize codon usage, lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis.</p>
  
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<h2 style="color: blue;">References</h2>
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<p><ul><i>
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===Source===
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<li>UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry</li>
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<li>Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/</li>
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Uniprot P38488 (https://www.uniprot.org/uniprotkb/P38488/entry)
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</i></ul></p>
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===References===
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Latest revision as of 14:27, 29 September 2024


Creatinase from Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 225
    Illegal BsaI site found at 1012


Sources

The codon-optimized nucleotide sequence from Pseudomonas putida adapted to Pseudomonas putida was obtained using the Codon Optimizer Tool from Integrated DNA Technologies. This step permits optimize codon usage, lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis.

References

  • UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry
  • Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/