Difference between revisions of "Part:BBa K5108004:Design"
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<h2 style="color: blue;">Sources</h2> | <h2 style="color: blue;">Sources</h2> | ||
− | <p>The codon optimized | + | <p>The codon-optimized nucleotidi sequence from <i>Pseudomonas putida</i> adapted to <i>Pseudomonas putida</i> was obtained using the <a href="https://eu.idtdna.com/pages/tools/codon-optimization-tool?returnurl=%2FCodonOpt" target="blank">Codon Optimizer Tool</a> from Integrated DNA Technologies. This step permits optimize codon usage, lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis.</p> |
<h2 style="color: blue;">References</h2> | <h2 style="color: blue;">References</h2> |
Latest revision as of 14:22, 29 September 2024
Creatinine amidohydrolase form Pseudomonas putida
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 61
Illegal NgoMIV site found at 663 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 199
Illegal BsaI.rc site found at 547
Sources
The codon-optimized nucleotidi sequence from Pseudomonas putida adapted to Pseudomonas putida was obtained using the Codon Optimizer Tool from Integrated DNA Technologies. This step permits optimize codon usage, lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis.
References
- UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry
- Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151