Difference between revisions of "Part:BBa K5108004:Design"
 
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<partinfo>BBa_K5108008 short</partinfo>
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<partinfo>BBa_K5108004 short</partinfo>
  
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<partinfo>BBa_K5108008 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K5108004 SequenceAndFeatures</partinfo>
  
  
 
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<h2 style="color: blue;">Sources</h2>
 
<h2 style="color: blue;">Sources</h2>
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<p>The codon optimized nucleotidic sequence from <i>Pseudomonas putida</i> adapted to <i>Pseudomonas putida</i> is obtained thanks to the Codon Optimization Tool from Integrated DNA Technologies <a href="https://eu.idtdna.com/pages/tools/codon-optimization-tool?returnurl=%2FCodonOpt" target="blank">codon optimizer tool.</a>. This step permits to lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis</p>
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<p>The codon-optimized nucleotidi sequence from <i>Pseudomonas putida</i> adapted to <i>Pseudomonas putida</i> was obtained using the <a href="https://eu.idtdna.com/pages/tools/codon-optimization-tool?returnurl=%2FCodonOpt" target="blank">Codon Optimizer Tool</a> from Integrated DNA Technologies. This step permits optimize codon usage, lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis.</p>
  
 
<h2 style="color: blue;">References</h2>
 
<h2 style="color: blue;">References</h2>
 
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<li>Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073</li>
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<li>UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry</li>
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<li>Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073</li>
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<li>Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151</li>
 
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Latest revision as of 14:22, 29 September 2024


Creatinine amidohydrolase form Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199
    Illegal BsaI.rc site found at 547


Sources

The codon-optimized nucleotidi sequence from Pseudomonas putida adapted to Pseudomonas putida was obtained using the Codon Optimizer Tool from Integrated DNA Technologies. This step permits optimize codon usage, lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis.

References

  • UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry
  • Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151