Difference between revisions of "Part:BBa K5382136"

 
 
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The stem-loop sequence of gRNA (guide RNA) refers to the reverse repeating sequence in its structure, which can be folded back to form a secondary structure, that is, a stem-loop structure, due to the hydrogen bond pairing between complementary bases. In gRNA, this conserved sequence helps stabilize gRNA binding to Cas9 protein.
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The Cas9 handle, specifically the stem-loop sequence of the guide RNA (gRNA), refers to the palindromic sequence within its structure. This sequence is capable of folding back upon itself to form a secondary structure, namely a stem-loop configuration, due to the hydrogen bonding between complementary nucleotide bases. Within the gRNA, this conserved sequence facilitates the stabilization of the gRNA-Cas9 protein complex.
  
 
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Latest revision as of 14:19, 29 September 2024


Cas9 handle-reverse repeating sequence in gRNA

The Cas9 handle, specifically the stem-loop sequence of the guide RNA (gRNA), refers to the palindromic sequence within its structure. This sequence is capable of folding back upon itself to form a secondary structure, namely a stem-loop configuration, due to the hydrogen bonding between complementary nucleotide bases. Within the gRNA, this conserved sequence facilitates the stabilization of the gRNA-Cas9 protein complex.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]