Difference between revisions of "Part:BBa K5384007"

Revision as of 14:05, 29 September 2024

pPIC9K-His-DDDDK-Vg

VGLYCIN gene and DDDDK site were linked to plasmid pPIC9K after inserting VGLYCIN coding polypeptide gene from Pichia pastoris plasmid. We designed the new plasmid in the experiment, because we need to transform Pichia pastoris to express our target protein VGLYCIN, the plasmid pPIC9K-3His-DDDK-vg, which we constructed and used, has a replication origin of the Pichia pastoris plasmid, an AOX1 promotor promoter, an α-factor secretion signal replication signal, and a plasmid AOX1 terminator replication terminator, it is convenient to insert and express the target gene and screen out the recombinant. The Ori site of the Pichia pastoris plasmid was derived from the Pichia pastoris plasmid.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1121
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1121
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 142
Illegal XhoI site found at 1342
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1121
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1121
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5384007 short</partinfo>
-VGLYCIN gene and DDDDK site were linked to plasmid pPIC9K after inserting VGLYCIN coding polypeptide gene from Pichia pastoris plasmid. We designed the new plasmid in the experiment, because we need to transform Pichia pastoris to express our target protein VGLYCIN, the plasmid PIC9K-3His-DDDK-vg, which we constructed and used, has a replication origin of the Pichia pastoris plasmid, an AOX1 promotor promoter, an &#945;-factor secretion signal replication signal, and a plasmid AOX1 terminator replication terminator, it is convenient to insert and express the target gene and screen out the recombinant. The Ori site of the Pichia pastoris plasmid was derived from the Pichia pastoris plasmid.
+VGLYCIN gene and DDDDK site were linked to plasmid pPIC9K after inserting VGLYCIN coding polypeptide gene from Pichia pastoris plasmid. We designed the new plasmid in the experiment, because we need to transform Pichia pastoris to express our target protein VGLYCIN, the plasmid pPIC9K-3His-DDDK-vg, which we constructed and used, has a replication origin of the Pichia pastoris plasmid, an AOX1 promotor promoter, an &#945;-factor secretion signal replication signal, and a plasmid AOX1 terminator replication terminator, it is convenient to insert and express the target gene and screen out the recombinant. The Ori site of the Pichia pastoris plasmid was derived from the Pichia pastoris plasmid.
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