Difference between revisions of "Part:BBa K211002:Design"

Latest revision as of 22:09, 21 October 2009

RI7-odr10 chimeric GPCR

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 761
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Designed with PCR. Primer Sequence

P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC

P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG

P3 RI7-N R GAGAAGAA ATACATGGGTTTGTGGAG

P4 Odr-10 F CCATGTAT TTCTTCTCAGTATTCTCG

P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG

P6 RI7-C F GATCATT TACTGCTTGCGCAACC

BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC

BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC

We would like to thank Professor Piali Sengupta, Department of Biology, Brandeis University for the kind gift of the odr-10 cDNA, and Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.

Source

Caenorhabditis elegans olfactory receptor Odr-10 (GenBank: U49449.1)

Rat olfactory protein, clone I7 (GenBank: M64386.1)

References

Radhika, V. et al . 2007. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 : 325–330

@@ Line 9: / Line 9: @@
 Designed with PCR.
 [[Image:primer design.jpg]]
-Primer	Sequence *
+Primer	Sequence
 P1	RI7-N F	        ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC
@@ Line 20: / Line 21: @@
 P5	Odr-10 R	GCAGTA AATGATCAGAATCAGAATGAG
 P6	RI7-C F	        GATCATT TACTGCTTGCGCAACC
 BioBrick F	ATATCT TCTAGATGGAACGAAGGAACCAC
-BioBrick R	GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC
+BioBrick R	GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC