Difference between revisions of "Part:BBa K211002:Design"

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===Design Notes===
 
===Design Notes===
  
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Designed with PCR.
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[[Image:primer design.jpg]]
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Primer Sequence
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P1 RI7-N F         ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC
  
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P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG
  
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P3 RI7-N R         GAGAAGAA ATACATGGGTTTGTGGAG
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P4 Odr-10 F CCATGTAT TTCTTCTCAGTATTCTCG
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P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG
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P6 RI7-C F         GATCATT TACTGCTTGCGCAACC                                                             
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BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC 
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BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC                                                         
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We would like to thank Professor Piali Sengupta, Department of Biology, Brandeis University for the kind gift of the odr-10 cDNA, and Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.
  
 
===Source===
 
===Source===
  
 
Caenorhabditis elegans olfactory receptor Odr-10 (GenBank: U49449.1)
 
Caenorhabditis elegans olfactory receptor Odr-10 (GenBank: U49449.1)
 +
 
Rat olfactory protein, clone I7 (GenBank: M64386.1)
 
Rat olfactory protein, clone I7 (GenBank: M64386.1)
  
 
===References===
 
===References===
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Radhika, V. ''et al'' . 2007. Chemical sensing of DNT by engineered olfactory yeast strain. ''Nat. Chem. Biol.'' '''3''' : 325–330

Latest revision as of 22:09, 21 October 2009

RI7-odr10 chimeric GPCR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 761
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with PCR. Primer design.jpg Primer Sequence

P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC

P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG

P3 RI7-N R GAGAAGAA ATACATGGGTTTGTGGAG

P4 Odr-10 F CCATGTAT TTCTTCTCAGTATTCTCG

P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG

P6 RI7-C F GATCATT TACTGCTTGCGCAACC


BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC

BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC


We would like to thank Professor Piali Sengupta, Department of Biology, Brandeis University for the kind gift of the odr-10 cDNA, and Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.

Source

Caenorhabditis elegans olfactory receptor Odr-10 (GenBank: U49449.1)

Rat olfactory protein, clone I7 (GenBank: M64386.1)

References

Radhika, V. et al . 2007. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 : 325–330