Difference between revisions of "Part:BBa K5280401"

 
 
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<partinfo>BBa_K5280401 short</partinfo>
 
<partinfo>BBa_K5280401 short</partinfo>
  
 
PcolE48 is a promoter to which LexRO (BBa_K5280129) binds and represses the expression of genes driven by the promoter. The promoter mainly consists of two parts: ColE promoter, which is a strong promoter, and LexA(408)-operator, which is the binding site of LexRO.
 
PcolE48 is a promoter to which LexRO (BBa_K5280129) binds and represses the expression of genes driven by the promoter. The promoter mainly consists of two parts: ColE promoter, which is a strong promoter, and LexA(408)-operator, which is the binding site of LexRO.
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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=== Sequence and Features ===
 
<partinfo>BBa_K5280401 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5280401 SequenceAndFeatures</partinfo>
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<!-- Add more about the biology of this part here -->
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===Usage and Biology===
 +
 +
PcolE408 basically has 2 parts:
 +
* ColE promoter, which is used to drive the expression of downstream genes;
 +
* LexA(408) operator, which is used to provide the regulatory site for LexRO.
 +
 +
The basic working principle is illustrated in the figure below. For more information, you can refer to the part page <partinfo>BBa_K5280129</partinfo>.
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<html>
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<body>
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    <div class="figure-box">
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        <img
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            src="https://static.igem.wiki/teams/5280/registry/lexro/lexro-mechanism-ver-2.png"
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            style="width:900px; display:block; margin:0;"
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            alt="In the experiments, E. coli transformed with an empty vector were used as negative controls."
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        >
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        <p>
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            This figure shows the mechanism of the eLightOn system (LexRO based regulation system).
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        </p>
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    </div>
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</body>
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</html>
  
  

Latest revision as of 13:56, 29 September 2024


PcolE408

PcolE48 is a promoter to which LexRO (BBa_K5280129) binds and represses the expression of genes driven by the promoter. The promoter mainly consists of two parts: ColE promoter, which is a strong promoter, and LexA(408)-operator, which is the binding site of LexRO.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

PcolE408 basically has 2 parts:

  • ColE promoter, which is used to drive the expression of downstream genes;
  • LexA(408) operator, which is used to provide the regulatory site for LexRO.

The basic working principle is illustrated in the figure below. For more information, you can refer to the part page BBa_K5280129.

In the experiments, E. coli transformed with an empty vector were used as negative controls.

This figure shows the mechanism of the eLightOn system (LexRO based regulation system).