Difference between revisions of "Part:BBa K211001:Design"
(→Design Notes) |
(→Design Notes) |
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Designed with PCR. | Designed with PCR. | ||
− | Primer design: | + | Primer design: |
− | + | ||
+ | P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC | ||
P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG | P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG | ||
BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC | BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC |
Revision as of 22:05, 21 October 2009
RI7
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 428
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 834
Design Notes
Designed with PCR.
Primer design:
P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC
We would like to thank Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.
Source
Rat olfactory protein, clone I7 (GenBank: M64386.1)
References
1.Radhika, V. et al . Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 , 325–330 (2007.)
2.Minic, J. et al. Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening. FEBS J. 272, 524–537 (2005).