Difference between revisions of "Part:BBa K5416001"

 
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<partinfo>BBa_K5416001 short</partinfo>
 
<partinfo>BBa_K5416001 short</partinfo>
  
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This basic part is a variant of HRT2trunc ([[Part:BBa_K5416000|BBa_K5416000]]), and codes for a truncated version of HRT2 rubber synthase enzyme ([[Part:BBa_K1088016|BBa_K1088016]]). Comparing to HRT2trunc, the His-tag has been replaced with a P22 scaffold protein ([[Part:BBa_K3187021|BBa_K3187021]]) to mediate binding towards the inner surface of P22 bacterial phage virus-like-particle (VLP).
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<html>
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  <img src="https://static.igem.wiki/teams/5416/logo-name-t.png"  alt="Description of image" width="200" />
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  <figcaption><strong>Imperial-College 2024</strong></figcaption>
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</figure>
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<p>
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This part is designed by Team Imperial_College in iGEM 2024. It is reported to form an artificial organelle which serves as an enclosed chamber for natrual rubber production.
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</p></html>
  
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<!-- Add more about the biology of this part here
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This basic part is a variant of HRT2trunc ([[Part:BBa_K5416000|BBa_K5416000]]), and codes for a truncated version of HRT2 rubber synthase enzyme ([[Part:BBa_K1088016|BBa_K1088016]]). Comparing to HRT2trunc, the His-tag has been replaced with a P22 scaffold protein ([[Part:BBa_K3187021|BBa_K3187021]]) to mediate binding towards the inner surface of P22 bacterial phage virus-like-particle (VLP).<br>
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===Usage and Biology===
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<b><i>Please be advised this part works the best as a composite part: [[Part:BBa_K5416060|BBa_K5416060]]</b></i>
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We have shown this part to have the following functions:
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<b>
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*Produces Rubber in small quantity
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*Interacts with P22 VLP capsid to be encapsulated
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</b>
  
 
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Revision as of 13:17, 29 September 2024


HRT2-SP

Description of image

Imperial-College 2024

This part is designed by Team Imperial_College in iGEM 2024. It is reported to form an artificial organelle which serves as an enclosed chamber for natrual rubber production.

This basic part is a variant of HRT2trunc (BBa_K5416000), and codes for a truncated version of HRT2 rubber synthase enzyme (BBa_K1088016). Comparing to HRT2trunc, the His-tag has been replaced with a P22 scaffold protein (BBa_K3187021) to mediate binding towards the inner surface of P22 bacterial phage virus-like-particle (VLP).
Please be advised this part works the best as a composite part: BBa_K5416060</b>

We have shown this part to have the following functions: <b>

  • Produces Rubber in small quantity
  • Interacts with P22 VLP capsid to be encapsulated

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]