Difference between revisions of "Part:BBa K5108003:Design"
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| − | <p>The codon optimized nucleotidic sequence from <i>Pseudomonas putida</i> adapted to <i>Pseudomonas putida</i> is obtained thanks to the Codon Optimization Tool from Integrated DNA Technologies <a href="https://eu.idtdna.com/pages/tools/codon-optimization-tool?returnurl=%2FCodonOpt" target="blank">codon optimizer tool | + | <p>The codon optimized nucleotidic sequence from <i>Pseudomonas putida</i> adapted to <i>Pseudomonas putida</i> is obtained thanks to the Codon Optimization Tool from Integrated DNA Technologies <a href="https://eu.idtdna.com/pages/tools/codon-optimization-tool?returnurl=%2FCodonOpt" target="blank">codon optimizer tool</a>. This step permits to lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis.</p> |
<h2 style="color: blue;">References</h2> | <h2 style="color: blue;">References</h2> | ||
Revision as of 13:15, 29 September 2024
Creatinase from Pseudomonas putida
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 225
Illegal BsaI site found at 1012
Sources
The codon optimized nucleotidic sequence from Pseudomonas putida adapted to Pseudomonas putida is obtained thanks to the Codon Optimization Tool from Integrated DNA Technologies codon optimizer tool. This step permits to lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis.
References
- UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry
- Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/