Difference between revisions of "Part:BBa I731012:Experience"

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Experiment : checking  BBa_I731012
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#Picked and cultured the following stab and glycerol stocks in 2mL of LB:
 +
##LB-Amp, BBa_I731012, (stab, received from iGEM)
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##LB-Amp+0.2%Glu, BBa_K084012(plac+rbs+LuxI(no LVA)), (XL10G)
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##LB-Amp+0.2%Glu, BBa_K084007(plac+rbs+LasI(no LVA)), (XL10G)
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#Pre-Cultured at 37°C for 12h.
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#Added 6.25% each of the pre-cultures to new LB medium.
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##LB-Amp, BBa_T9002, BBa_K084012(plac+rbs+LuxI(no LVA)), BBa_K084007(plac+rbs+LasI(no LVA))
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#Cultured at 37°C for 12h
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#Mixed the sender cells BBa_K084012 and BBa_K084007 both with BBa_I1731012 at a 1:1 ratio.
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##BBa_I1731012 alone.
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##BBa_K084012:BBa_I1731012=1:1
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##BBa_K084007:BBa_BBa_I1731012=1:1
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#Culture 12h and observe results(follow picture).
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*Funakoshi, UVGL-58 : long wave(365 nm)
 
<html><a href="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" width="400" height="300"></a></html>
 
<html><a href="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" width="400" height="300"></a></html>

Revision as of 22:04, 21 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I731012

User Reviews

UNIQ90f779bae3cfc61c-partinfo-00000000-QINU UNIQ90f779bae3cfc61c-partinfo-00000001-QINU Experiment : checking BBa_I731012

  1. Picked and cultured the following stab and glycerol stocks in 2mL of LB:
    1. LB-Amp, BBa_I731012, (stab, received from iGEM)
    2. LB-Amp+0.2%Glu, BBa_K084012(plac+rbs+LuxI(no LVA)), (XL10G)
    3. LB-Amp+0.2%Glu, BBa_K084007(plac+rbs+LasI(no LVA)), (XL10G)
  2. Pre-Cultured at 37°C for 12h.
  3. Added 6.25% each of the pre-cultures to new LB medium.
    1. LB-Amp, BBa_T9002, BBa_K084012(plac+rbs+LuxI(no LVA)), BBa_K084007(plac+rbs+LasI(no LVA))
  4. Cultured at 37°C for 12h
  5. Mixed the sender cells BBa_K084012 and BBa_K084007 both with BBa_I1731012 at a 1:1 ratio.
    1. BBa_I1731012 alone.
    2. BBa_K084012:BBa_I1731012=1:1
    3. BBa_K084007:BBa_BBa_I1731012=1:1
  6. Culture 12h and observe results(follow picture).
  • Funakoshi, UVGL-58 : long wave(365 nm)