Difference between revisions of "Part:BBa K5108006"
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<h2 style="color: blue;"> <b>Usage and Biology</b></h2>
 
<h2 style="color: blue;"> <b>Usage and Biology</b></h2>
  
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<p>Ribosome Binding Site of gene <i>aprA</i> of <i>P. fluorescens</i>. It was used for protein translation in <i>P. fluorescens</i> from [1].
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<p><a href="https://www.uniprot.org/uniprotkb/P83772/entry" target="blank">CrnA</a> is an enzyme that catalyzes the degradation of creatinine into creatine in <i>Pseudomonas putida</i>.  
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This RBS was synthesized in an operon with two genes and another RBS and cloned into a plasmid. After transformation in <i>P. fluorescens</i>, our team had successful protein expression with this RBS. Check out composite part <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>for more information.  
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In the context of our project, the gene encoding this enzyme (<i>crnA</i>) was synthesized in an operon with the gene for creatine degradation. You can find more information about our construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>.  
 
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<li>Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073</li>
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<li>UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry</li>
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<li>Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151</li>
 
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Revision as of 12:47, 29 September 2024

aprA RBS from Pseudomonas protegens (CHA0)


    Contents
  1. Usage and Biology
  2. References



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

CrnA is an enzyme that catalyzes the degradation of creatinine into creatine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (crnA) was synthesized in an operon with the gene for creatine degradation. You can find more information about our construct and our results in BBa_K5108009.


References

  1. UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry
  2. Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151
    3.