Difference between revisions of "Part:BBa K5387000:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K5387000 short</partinfo>
 
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<partinfo>BBa_K5387000 SequenceAndFeatures</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The sequence has been optimized for expression in E. coli.  
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The sequence has been optimized for expression in ''E. coli''.  
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The sequence contains the amino acid sequence "QPPP", which is very difficult for the ribosomes in E. coli to translate. We did not do it, but for future projects we would recommend altering this part of the sequence, either by mutation or when ordering the sequence.
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===Source===
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This gene is expressed in humans.
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The vector used was pET-28a(+), which His-ag we utilized when expressing our protein.  
  
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===References===
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Primers for cloning into pET-28a(+) between restriction sites NdeI and EcoRI:
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: fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
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: rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'

Latest revision as of 10:31, 29 September 2024

Design Notes

The sequence has been optimized for expression in E. coli.

The vector used was pET-28a(+), which His-ag we utilized when expressing our protein.

Primers for cloning into pET-28a(+) between restriction sites NdeI and EcoRI:

fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'