Difference between revisions of "Part:BBa K196003"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K196003 short</partinfo>
 
<partinfo>BBa_K196003 short</partinfo>
  
−
Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. Here you have HfsH.
+
<i>Caulobacter crescentus</i> is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly composed of polysaccharide. Different proteins are required in the holdfast synthesis, export and attachment in <i>C.crescentus</i> (for more details, please refer to the "introduction" section). In our project, we wanted to implement the glue production in <i>Escherichia coli</i>. As <i>E. coli</i> possesses homolog proteins, we decid to insert the hfsG and hfsH genes in a plasmid. HfsG [https://parts.igem.org/Part:BBa_K196002:Design] is a glycosyltransferase and HfsH is a carbohydrate esterase. Here is the BB for the hfsH gene
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:46, 21 October 2009

HfsH protein from Caulobacter crescentus

Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly composed of polysaccharide. Different proteins are required in the holdfast synthesis, export and attachment in C.crescentus (for more details, please refer to the "introduction" section). In our project, we wanted to implement the glue production in Escherichia coli. As E. coli possesses homolog proteins, we decid to insert the hfsG and hfsH genes in a plasmid. HfsG [1] is a glycosyltransferase and HfsH is a carbohydrate esterase. Here is the BB for the hfsH gene

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]