Difference between revisions of "Part:BBa K5311006"
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By breaking down the peptidoglycan, the endolysin disrupts the rigid structure of the cell wall, leading to a loss of osmotic integrity. As a result, the bacterial cell can no longer withstand the internal pressure, causing rapid cell lysis. This mechanism is particularly effective against Gram-positive bacteria like C. acnes because they have a thick peptidoglycan layer that is more accessible to endolysins, unlike Gram-negative bacteria, which possess an additional outer membrane that restricts endolysin access.[1] | By breaking down the peptidoglycan, the endolysin disrupts the rigid structure of the cell wall, leading to a loss of osmotic integrity. As a result, the bacterial cell can no longer withstand the internal pressure, causing rapid cell lysis. This mechanism is particularly effective against Gram-positive bacteria like C. acnes because they have a thick peptidoglycan layer that is more accessible to endolysins, unlike Gram-negative bacteria, which possess an additional outer membrane that restricts endolysin access.[1] | ||
| + | |||
| + | <p><strong>Advantages of Endolysins Against <em>C. acnes</em></strong>:</p> | ||
| + | <ul> | ||
| + | <li><strong>High Specificity:</strong> Endolysins are typically highly specific to their target bacteria, minimizing the risk of disrupting the beneficial skin microbiota.</li> | ||
| + | <li><strong>Low Risk of Resistance:</strong> Unlike traditional antibiotics, endolysins act on essential and conserved structural components of the bacterial cell wall, making it difficult for bacteria to develop resistance.</li> | ||
| + | <li><strong>Direct Bacterial Destruction:</strong> In contrast to other treatments that only inhibit bacterial growth, endolysins cause direct and rapid bacterial cell death, making them a potent tool for treating <em>C. acnes</em>-associated infections.</li> | ||
| + | </ul> | ||
Revision as of 09:53, 29 September 2024
CAP 10-3 Endolysin
Sequencing
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 266
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 34
Illegal NgoMIV site found at 445 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 193
Biology
In the case of the endolysin derived from the CAP 10-3 bacteriophage, its action specifically targets the degradation of the cell wall of Cutibacterium acnes, a Gram-positive bacterium with a cell wall rich in peptidoglycan, which is a crucial structural component. This endolysin is classified as an N-acetylmuramoyl-L-alanine amidase, which cleaves the bond between the N-acetylmuramic acid and the L-alanine residue in the peptidoglycan backbone.
By breaking down the peptidoglycan, the endolysin disrupts the rigid structure of the cell wall, leading to a loss of osmotic integrity. As a result, the bacterial cell can no longer withstand the internal pressure, causing rapid cell lysis. This mechanism is particularly effective against Gram-positive bacteria like C. acnes because they have a thick peptidoglycan layer that is more accessible to endolysins, unlike Gram-negative bacteria, which possess an additional outer membrane that restricts endolysin access.[1]
Advantages of Endolysins Against C. acnes:
- High Specificity: Endolysins are typically highly specific to their target bacteria, minimizing the risk of disrupting the beneficial skin microbiota.
- Low Risk of Resistance: Unlike traditional antibiotics, endolysins act on essential and conserved structural components of the bacterial cell wall, making it difficult for bacteria to develop resistance.
- Direct Bacterial Destruction: In contrast to other treatments that only inhibit bacterial growth, endolysins cause direct and rapid bacterial cell death, making them a potent tool for treating C. acnes-associated infections.