Difference between revisions of "Part:BBa K5034225:Design"
Line 12: Line 12:
 
===Source===
 
===Source===
  
−
Inorganic polyphosphate/ATP-NAD kinase(PPNK) from Mycobacterium tuberculosis H37Rv. NCBI reference sequence: NC_000962.3:1918746-1919669
+
Inorganic polyphosphate/ATP-NAD kinase(PPNK) from <i>Mycobacterium tuberculosis H37Rv</i>. NCBI reference sequence: NC_000962.3:1918746-1919669
  
 
===References===
 
===References===
  
 
Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004
 
Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004

Revision as of 07:32, 29 September 2024


Poly P -> NADP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 11
    Illegal PstI site found at 3787
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 11
    Illegal PstI site found at 3787
    Illegal NotI site found at 2828
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 11
    Illegal BglII site found at 3574
    Illegal XhoI site found at 4985
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 11
    Illegal PstI site found at 3787
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 11
    Illegal PstI site found at 3787
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 4238
    Illegal NgoMIV site found at 4521
    Illegal AgeI site found at 396
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3150
    Illegal SapI.rc site found at 4087
    Illegal SapI.rc site found at 4297


Design Notes

PAP gene was PCR-amplified and inserted into the plasmid pYYDT(after NdeI and XhoI digestion) to get the expression vector. It then got transformed into E. coli DH5α to amplify, and was purified by picking monoclonal culture and sequencing.

Source

Inorganic polyphosphate/ATP-NAD kinase(PPNK) from Mycobacterium tuberculosis H37Rv. NCBI reference sequence: NC_000962.3:1918746-1919669

References

Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004