Difference between revisions of "Part:BBa K209474"

 
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<partinfo>BBa_K209474 short</partinfo>
 
<partinfo>BBa_K209474 short</partinfo>
  
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Stop codons, flanked by AarI CD sites.
  
  
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===Usage and Biology===
 
===Usage and Biology===
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The purpose of this part is to insert stop codons in 3 different reading frames into the C-D position of an AarI fusion contruct.  The FKBP domain comes after the stop codons, and is only included to facilitate extraction and ligation of this part.
  
 
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Revision as of 21:21, 21 October 2009

AarI C-D part, 3xSTOP-FKBP

Stop codons, flanked by AarI CD sites.


Usage and Biology

The purpose of this part is to insert stop codons in 3 different reading frames into the C-D position of an AarI fusion contruct. The FKBP domain comes after the stop codons, and is only included to facilitate extraction and ligation of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3
  • 1000
    COMPATIBLE WITH RFC[1000]