Difference between revisions of "Part:BBa K245116:Design"
 
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===Design Notes===
 
===Design Notes===
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This part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37.
+
This part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. Part contains the coding region for the 24 kDa N-terminal segment of gyrase B of E.coli.
  
  
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===Source===
 
===Source===
  
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the part was multiplied using PCR
+
The part was multiplied by PCR from GyrB-L20-MD2/pFLAG-CMV3.
  
 
===References===
 
===References===

Latest revision as of 21:20, 21 October 2009

GyrB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 238
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. Part contains the coding region for the 24 kDa N-terminal segment of gyrase B of E.coli.


Source

The part was multiplied by PCR from GyrB-L20-MD2/pFLAG-CMV3.

References