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         <img src="https://static.igem.wiki/teams/5351/bba-k5351010/1.png" width="50%" alt="Figure 1. Plasmid map of X-3-XI-2-gRNA-Backbone">
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         <img src="https://static.igem.wiki/teams/5351/bba-k5351010/1.png" width="40%" alt="Figure 1. Plasmid map of X-3-XI-2-gRNA-Backbone">
 
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             <caption>Figure 1. Plasmid map of X-3-XI-2-gRNA-Backbone</caption>
 
             <caption>Figure 1. Plasmid map of X-3-XI-2-gRNA-Backbone</caption>

Latest revision as of 06:47, 29 September 2024


X-3-XI-2-gRNA-Backbone



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal XbaI site found at 1140
    Illegal XbaI site found at 1417
    Illegal XbaI site found at 1582
    Illegal XbaI site found at 1874
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 4131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal NheI site found at 1165
    Illegal NheI site found at 1607
    Illegal NheI site found at 3850
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 4131
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal BamHI site found at 1428
    Illegal XhoI site found at 1862
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal XbaI site found at 1140
    Illegal XbaI site found at 1417
    Illegal XbaI site found at 1582
    Illegal XbaI site found at 1874
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 4131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal XbaI site found at 1140
    Illegal XbaI site found at 1417
    Illegal XbaI site found at 1582
    Illegal XbaI site found at 1874
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 4131
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3027
    Illegal SapI site found at 1944


X-3-XI-2-gRNA-Backbone Documentation

Origin

Organism: E.coli; synthesized

Properties

The X-3-XI-2-gRNA-Backbone is a plasmid backbone used for linking relevant genes.

Figure 1. Plasmid map of X-3-XI-2-gRNA-Backbone
Figure 1. Plasmid map of X-3-XI-2-gRNA-Backbone